Paschalis Kratsios

Assistant Professor
Research Summary
My lab uses the specific strengths of two model systems (C.elegans and mice) to reveal the gene regulatory mechanisms that control motor neuron development and function. To reveal such mechanisms we employ novel methodology, such as whole genome sequencing, CRISPR genome editing, ATAC-seq and cell type-specific transcriptome profiling. Our laboratory aims to systematically test whether the function of the gene regulatory factors we discover in C.elegans is conserved across phylogeny using mouse genetics and novel genomic approaches. A detailed understanding of how the motor neurons develop and function may provide novel entry points into the etiology, diagnosis or treatment of motor neuron disorders, such as spinal muscular atrophy (SMA) and amyotrophic lateral sclerosis (ALS). From the basic science perspective, our research will reveal novel transcription factors, their targets and the cis-regulatory elements (motifs) through which these factors act. Such decoding of cis-regulatory information is a vital step toward understanding genome function.
neuronal development, gene regulation, C.elegans genetics, mouse genetics, Transcription Factors, chromatin factors, Gene Regulatory Networks
  • European Molecular Biology Laboratory (EMBL), Ph.D. Developmental Biology 05/2009
  • Columbia University, Postdoctoral training Developmental Neurobiology 02/2016
Awards & Honors
  • 2013 - 2018 K99/R00 Pathway to Independence Award National Institute of Health
  • 2017 - 2019 Award for Basic Research in Neurobiology Whitehall Foundation
  1. Sonobe Y, Aburas J, Krishnan G, Fleming AC, Ghadge G, Islam P, Warren EC, Gu Y, Kankel MW, Brown AEX, Kiskinis E, Gendron TF, Gao FB, Roos RP, Kratsios P. A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation. Nat Commun. 2021 Oct 15; 12(1):6025. View in: PubMed

  2. Li Y, Kratsios P. Transgenic reporter analysis of ChIP-Seq-defined enhancers identifies novel target genes for the terminal selector UNC-3/Collier/Ebf. MicroPubl Biol. 2021; 2021. View in: PubMed

  3. Chakraborty K, Anees P, Surana S, Martin S, Aburas J, Moutel S, Perez F, Koushika SP, Kratsios P, Krishnan Y. Tissue-specific targeting of DNA nanodevices in a multicellular living organism. Elife. 2021 07 28; 10. View in: PubMed

  4. Li Y, Osuma A, Correa E, Okebalama MA, Dao P, Gaylord O, Aburas J, Islam P, Brown AE, Kratsios P. Establishment and maintenance of motor neuron identity via temporal modularity in terminal selector function. Elife. 2020 10 01; 9. View in: PubMed

  5. Serrano-Saiz E, Gulez B, Pereira L, Gendrel M, Kerk SY, Vidal B, Feng W, Wang C, Kratsios P, Rand JB, Hobert O. Modular Organization of Cis-regulatory Control Information of Neurotransmitter Pathway Genes in Caenorhabditis elegans. Genetics. 2020 07; 215(3):665-681. View in: PubMed

  6. Feng W, Li Y, Dao P, Aburas J, Islam P, Elbaz B, Kolarzyk A, Brown AE, Kratsios P. A terminal selector prevents a Hox transcriptional switch to safeguard motor neuron identity throughout life. Elife. 2020 01 03; 9. View in: PubMed

  7. Catela C, Kratsios P. Transcriptional mechanisms of motor neuron development in vertebrates and invertebrates. Dev Biol. 2021 07; 475:193-204. View in: PubMed

  8. Hobert O, Kratsios P. Neuronal identity control by terminal selectors in worms, flies, and chordates. Curr Opin Neurobiol. 2019 06; 56:97-105. View in: PubMed

  9. Catela C, Correa E, Wen K, Aburas J, Croci L, Consalez GG, Kratsios P. An ancient role for collier/Olf/Ebf (COE)-type transcription factors in axial motor neuron development. Neural Dev. 2019 01 18; 14(1):2. View in: PubMed

  10. Kratsios P, Hobert O. Nervous System Development: Flies and Worms Converging on Neuron Identity Control. Curr Biol. 2018 10 08; 28(19):R1154-R1157. View in: PubMed

  11. Kratsios P, Kerk SY, Catela C, Liang J, Vidal B, Bayer EA, Feng W, De La Cruz ED, Croci L, Consalez GG, Mizumoto K, Hobert O. An intersectional gene regulatory strategy defines subclass diversity of C. elegans motor neurons. Elife. 2017 07 05; 6. View in: PubMed

  12. Kerk SY, Kratsios P, Hart M, Mourao R, Hobert O. Diversification of C. elegans Motor Neuron Identity via Selective Effector Gene Repression. Neuron. 2017 Jan 04; 93(1):80-98. View in: PubMed

  13. Pereira L, Kratsios P, Serrano-Saiz E, Sheftel H, Mayo AE, Hall DH, White JG, LeBoeuf B, Garcia LR, Alon U, Hobert O. A cellular and regulatory map of the cholinergic nervous system of C. elegans. Elife. 2015 Dec 25; 4. View in: PubMed

  14. Kratsios P, Pinan-Lucarré B, Kerk SY, Weinreb A, Bessereau JL, Hobert O. Transcriptional coordination of synaptogenesis and neurotransmitter signaling. Curr Biol. 2015 May 18; 25(10):1282-95. View in: PubMed

  15. Kratsios P, Stolfi A, Levine M, Hobert O. Coordinated regulation of cholinergic motor neuron traits through a conserved terminal selector gene. Nat Neurosci. 2011 Nov 27; 15(2):205-14. View in: PubMed

  16. Tursun B, Patel T, Kratsios P, Hobert O. Direct conversion of C. elegans germ cells into specific neuron types. Science. 2011 Jan 21; 331(6015):304-8. View in: PubMed

  17. Catela C, Kratsios P, Hede M, Lang F, Rosenthal N. Serum and glucocorticoid-inducible kinase 1 (SGK1) is necessary for vascular remodeling during angiogenesis. Dev Dyn. 2010 Aug; 239(8):2149-60. View in: PubMed

  18. Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F. Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart. Circ Res. 2010 Feb 19; 106(3):559-72. View in: PubMed

  19. Kratsios P, Huth M, Temmerman L, Salimova E, Al Banchaabouchi M, Sgoifo A, Manghi M, Suzuki K, Rosenthal N, Mourkioti F. Antioxidant amelioration of dilated cardiomyopathy caused by conditional deletion of NEMO/IKKgamma in cardiomyocytes. Circ Res. 2010 Jan 08; 106(1):133-44. View in: PubMed

  20. Catela C, Bilbao-Cortes D, Slonimsky E, Kratsios P, Rosenthal N, Te Welscher P. Multiple congenital malformations of Wolf-Hirschhorn syndrome are recapitulated in Fgfrl1 null mice. Dis Model Mech. 2009 May-Jun; 2(5-6):283-94. View in: PubMed

  21. Platsoucas CD, Lu S, Nwaneshiudu I, Solomides C, Agelan A, Ntaoula N, Purev E, Li LP, Kratsios P, Mylonas E, Jung WJ, Evans K, Roberts S, Lu Y, Layvi R, Lin WL, Zhang X, Gaughan J, Monos DS, Oleszak EL, White JV. Abdominal aortic aneurysm is a specific antigen-driven T cell disease. Ann N Y Acad Sci. 2006 Nov; 1085:224-35. View in: PubMed

  22. Mourkioti F, Kratsios P, Luedde T, Song YH, Delafontaine P, Adami R, Parente V, Bottinelli R, Pasparakis M, Rosenthal N. Targeted ablation of IKK2 improves skeletal muscle strength, maintains mass, and promotes regeneration. J Clin Invest. 2006 Nov; 116(11):2945-54. View in: PubMed